Lactobacillus Plantarum for Human Life: Molecular Approach
DOI:
https://doi.org/10.56778/rjslr.v3i2.576Keywords:
Lactobacillus plantarum, SCFA, Nrf2, free radicals, and probioticAbstract
Lactobacillus plantarum (L. plantarum) increases short-chain fatty acids (SCFA) levels in the intestines, which acts as a signal to activate the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway. This activation is crucial for reducing oxidative stress and maintaining mitochondrial balance. This study aims to review several research papers that explore and highlight how L. plantarum makes SCFAs to activate Nrf2, specifically the strategies it uses to combat free radicals. The systematic review technique systematically investigates, assesses, analyzes, and interprets all study findings relevant to the research topic. We sourced articles from three databases: Scopus, Web of Science, and Google Scholar, covering the period from 2014 to 2024. The study found that SCFAs made by L. plantarum are important signals that trigger the Nrf2 signaling pathway, which helps protect cells from damage caused by oxidation and inflammation. L. plantarum is a beneficial probiotic that uses SCFAs to trigger the Nrf2 pathway, providing strong protection against free radicals and supporting various health benefits.
References
Aboulgheit, A., Karbasiafshar, C., Zhang, Z., Sabra, M., Shi, G., Tucker, A., Sodha, N., Abid, M. R., & Sellke, F. W. (2021). Lactobacillus plantarum probiotic induces Nrf2-mediated antioxidant signaling and eNOS expression resulting in improvement of myocardial diastolic function. American Journal of Physiology-Heart and Circulatory Physiology, 321(5), H839–H849. https://doi.org/10.1152/ajpheart.00278.2021
Angioi, M., & Hiller, C. E. (2023). Systematic Literature Reviews. In M. Angioi & C. E. Hiller, Research Methods in the Dance Sciences (pp. 265–280). University Press of Florida. https://doi.org/10.5744/florida/9780813069548.003.0018
Behera, S. S., Ray, R. C., & Zdolec, N. (2018). Lactobacillus plantarum with Functional Properties: An Approach to Increase Safety and Shelf-Life of Fermented Foods. BioMed Research International, 2018, 1–18. https://doi.org/10.1155/2018/9361614
Belicová, A., Mikulášová, M., & Dušinský, R. (2013). Probiotic Potential and Safety Properties of Lactobacillus plantarum from Slovak Bryndza Cheese. BioMed Research International, 2013, 1–8. https://doi.org/10.1155/2013/760298
Beltrán-Velasco, A. I., Reiriz, M., Uceda, S., & Echeverry-Alzate, V. (2024). Lactiplantibacillus (Lactobacillus) plantarum as a Complementary Treatment to Improve Symptomatology in Neurodegenerative Disease: A Systematic Review of Open Access Literature. International Journal of Molecular Sciences, 25(5), 3010. https://doi.org/10.3390/ijms25053010
Chen, Q., Liu, C., Zhang, Y., Wang, S., & Li, F. (2022). Effect of Lactobacillus plantarum KSFY01 on the exercise capacity of D-galactose-induced oxidative stress-aged mice. Frontiers in Microbiology, 13, 1030833. https://doi.org/10.3389/fmicb.2022.1030833
Corsetti, A., & Valmorri, S. (2011). Lactic Acid Bacteria | Lactobacillus spp.: Lactobacillus plantarum. In Encyclopedia of Dairy Sciences (pp. 111–118). Elsevier. https://doi.org/10.1016/B978-0-12-374407-4.00263-6
Di Chiano, M., Rocchetti, M. T., Spano, G., Russo, P., Allegretta, C., Milior, G., Gadaleta, R. M., Sallustio, F., Pontrelli, P., Gesualdo, L., Avolio, C., Fiocco, D., & Gallone, A. (2024). Lactobacilli Cell-Free Supernatants Modulate Inflammation and Oxidative Stress in Human Microglia via NRF2-SOD1 Signaling. Cellular and Molecular Neurobiology, 44(1), 60. https://doi.org/10.1007/s10571-024-01494-1
Dinev, T., Beev, G., Tzanova, M., Denev, S., Dermendzhieva, D., & Stoyanova, A. (2018). Antimicrobial activity of Lactobacillus plantarum against pathogenic and food spoilage microorganisms: A review. Bulgarian Journal of Veterinary Medicine, 21(3), 253–268. https://doi.org/10.15547/bjvm.1084
Dong, J., Ping, L., Xie, Q., Liu, D., Zhao, L., Evivie, S. E., Wang, Z., Li, B., & Huo, G. (2022). Lactobacillus plantarum KLDS1.0386 with antioxidant capacity ameliorates the lipopolysaccharide-induced acute liver injury in mice by NF-κB and Nrf2 pathway. Food Bioscience, 47, 101589. https://doi.org/10.1016/j.fbio.2022.101589
Du, G., Guo, Q., Qiang, L., Chang, S., Yan, X., Chen, H., Yuan, Y., & Yue, T. (2023). Influence of encapsulated Lactobacillus plantarum and eugenol on the physicochemical properties and microbial community of fresh-cut apples. Food Chemistry: X, 17, 100563. https://doi.org/10.1016/j.fochx.2023.100563
Dyah Kusumo, P., Maulahela, H., Pitarini Utari, A., Surono, I. S., Soebandrio, A., & Abdullah, M. (2019). Probiotic Lactobacillus plantarum IS 10506 supplementation increase SCFA of women with functional constipation. Iranian Journal of Microbiology. https://doi.org/10.18502/ijm.v11i5.1957
Gao, D., Gao, Z., & Zhu, G. (2013). Antioxidant effects of Lactobacillus plantarum via activation of transcription factor Nrf2. Food & Function, 4(6), 982. https://doi.org/10.1039/c3fo30316k
González-Bosch, C., Boorman, E., Zunszain, P. A., & Mann, G. E. (2021). Short-chain fatty acids as modulators of redox signaling in health and disease. Redox Biology, 47, 102165. https://doi.org/10.1016/j.redox.2021.102165
Gupta, N., El-Gawaad, N. S. A., Mallasiy, L. O., Gupta, H., Yadav, V. K., Alghamdi, S., & Qusty, N. F. (2024). Microbial dysbiosis and the aging process: A review on the potential age-deceleration role of Lactiplantibacillus plantarum. Frontiers in Microbiology, 15, 1260793. https://doi.org/10.3389/fmicb.2024.1260793
Haddaway, N. R., Page, M. J., Pritchard, C. C., & McGuinness, L. A. (2022). PRISMA2020: An R package and Shiny app for producing PRISMA 2020‐compliant flow diagrams, with interactivity for optimised digital transparency and Open Synthesis. Campbell Systematic Reviews, 18(2), e1230. https://doi.org/10.1002/cl2.1230
Huang, W.-C., Wei, C.-C., Huang, C.-C., Chen, W.-L., & Huang, H.-Y. (2019). The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes. Nutrients, 11(2), 353. https://doi.org/10.3390/nu11020353
Ismael, M., Gu, Y., Cui, Y., Wang, T., Yue, F., Qin, Y., & Lü, X. (2022). Probiotic of Lactiplantibacillus plantarum NWAFU-BIO-BS29 Isolated from Chinese Traditional Fermented Milk and Its Potential Therapeutic Applications Based on Gut Microbiota Regulation. Foods, 11(23), 3766. https://doi.org/10.3390/foods11233766
Jiménez Soto, R. E., Vanegas Espinoza, J. I., Cárdenas Chacha, K. G., Macas González, L. A., & Jima Blacio, J. J. (2024). Implicaciones de Lactobacillus Plantarum en Trastornos Patológicos. Revisión Bibliográfica. Ciencia Latina Revista Científica Multidisciplinar, 8(4), 4959–4978. https://doi.org/10.37811/cl_rcm.v8i4.12721
Kaushik, J. K., Kumar, A., Duary, R. K., Mohanty, A. K., Grover, S., & Batish, V. K. (2009). Functional and Probiotic Attributes of an Indigenous Isolate of Lactobacillus plantarum. PLoS ONE, 4(12), e8099. https://doi.org/10.1371/journal.pone.0008099
Kim, H.-J., An, J., & Ha, E.-M. (2022). Lactobacillus plantarum-derived metabolites sensitize the tumor-suppressive effects of butyrate by regulating the functional expression of SMCT1 in 5-FU-resistant colorectal cancer cells. Journal of Microbiology, 60(1), 100–117. https://doi.org/10.1007/s12275-022-1533-1
Kong, Y., Olejar, K. J., On, S. L. W., & Chelikani, V. (2020). The Potential of Lactobacillus spp. For Modulating Oxidative Stress in the Gastrointestinal Tract. Antioxidants, 9(7), 610. https://doi.org/10.3390/antiox9070610
Lee, Y.-S., Lee, D., Park, G.-S., Ko, S.-H., Park, J., Lee, Y.-K., & Kang, J. (2021). Lactobacillus plantarum HAC01 ameliorates type 2 diabetes in high-fat diet and streptozotocin-induced diabetic mice in association with modulating the gut microbiota. Food & Function, 12(14), 6363–6373. https://doi.org/10.1039/D1FO00698C
Li, H., Cheng, S., Wang, Y., Sun, Y., Zhang, J., Sun, M., Man, C., Zhang, Y., & Jiang, Y. (2024a). Lactobacillus plantarum J26 alleviates alcohol-induced oxidative liver injury by regulating the Nrf2 signaling pathway. Food Science and Human Wellness, 13(4), 2068–2078. https://doi.org/10.26599/FSHW.2022.9250172
Li, H., Cheng, S., Wang, Y., Sun, Y., Zhang, J., Sun, M., Man, C., Zhang, Y., & Jiang, Y. (2024b). Lactobacillus plantarum J26 alleviates alcohol-induced oxidative liver injury by regulating the Nrf2 signaling pathway. Food Science and Human Wellness, 13(4), 2068–2078. https://doi.org/10.26599/FSHW.2022.9250172
Lin, X., Xia, Y., Wang, G., Xiong, Z., Zhang, H., Lai, F., & Ai, L. (2018). Lactobacillus plantarum AR501 Alleviates the Oxidative Stress of D‐Galactose‐Induced Aging Mice Liver by Upregulation of Nrf2‐Mediated Antioxidant Enzyme Expression. Journal of Food Science, 83(7), 1990–1998. https://doi.org/10.1111/1750-3841.14200
Liu, Y.-W., Liong, M.-T., & Tsai, Y.-C. (2018). New perspectives of Lactobacillus plantarum as a probiotic: The gut-heart-brain axis. Journal of Microbiology, 56(9), 601–613. https://doi.org/10.1007/s12275-018-8079-2
Long, L., Meng, X., Sun, J., Jing, L., Chen, D., & Yu, R. (2022). Ameliorated effect of Lactobacillus plantarum SCS2 on the oxidative stress in HepG2 cells induced by AFB1. Food Science and Technology, 42, e16522. https://doi.org/10.1590/fst.16522
Mayo, B., & Flórez, A. B. (2022). Lactic Acid Bacteria: Lactobacillus plantarum. In Encyclopedia of Dairy Sciences (pp. 206–217). Elsevier. https://doi.org/10.1016/B978-0-08-100596-5.00856-8
Minj, J., Chandra, P., Paul, C., & Sharma, R. K. (2021). Bio-functional properties of probiotic Lactobacillus: Current applications and research perspectives. Critical Reviews in Food Science and Nutrition, 61(13), 2207–2224. https://doi.org/10.1080/10408398.2020.1774496
Mu, G., Li, H., Tuo, Y., Gao, Y., & Zhang, Y. (2019). Antioxidative effect of Lactobacillus plantarum Y44 on 2,2′-azobis(2-methylpropionamidine) dihydrochloride (ABAP)-damaged Caco-2 cells. Journal of Dairy Science, 102(8), 6863–6875. https://doi.org/10.3168/jds.2019-16447
Ngo, V., & Duennwald, M. L. (2022). Nrf2 and Oxidative Stress: A General Overview of Mechanisms and Implications in Human Disease. Antioxidants, 11(12), 2345. https://doi.org/10.3390/antiox11122345
Oladejo, B. O., Akingbade, G. T., Adeboboye, C. F., & Adeoye, S. B. (2024). Lactobacillus species modulates TNF-α and Nrf2 expression in carrageenan-induced rat paw oedema. The Microbe, 2, 100050. https://doi.org/10.1016/j.microb.2024.100050
Olotu, T., & Ferrell, J. M. (2024). Lactobacillus sp. For the Attenuation of Metabolic Dysfunction-Associated Steatotic Liver Disease in Mice. Microorganisms, 12(12), 2488. https://doi.org/10.3390/microorganisms12122488
Prete, R., Long, S. L., Joyce, S. A., & Corsetti, A. (2020). Genotypic and phenotypic characterization of food-associated Lactobacillus plantarum isolates for potential probiotic activities. FEMS Microbiology Letters, 367(10), fnaa076. https://doi.org/10.1093/femsle/fnaa076
Qian, Y., Zhang, J., Zhou, X., Yi, R., Mu, J., Long, X., Pan, Y., Zhao, X., & Liu, W. (2018). Lactobacillus plantarum CQPC11 Isolated from Sichuan Pickled Cabbages Antagonizes d-galactose-Induced Oxidation and Aging in Mice. Molecules, 23(11), 3026. https://doi.org/10.3390/molecules23113026
Rezaie, N., Aghamohammad, S., Haj Agha Gholizadeh Khiavi, E., Khatami, S., Sohrabi, A., & Rohani, M. (2024). The comparative anti-oxidant and anti-inflammatory efficacy of postbiotics and probiotics through Nrf-2 and NF-kB pathways in DSS-induced colitis model. Scientific Reports, 14(1), 11560. https://doi.org/10.1038/s41598-024-62441-0
Rezgui, R., Walia, R., Sharma, J., Sidhu, D., Alshagadali, K., Ray Chaudhuri, S., Saeed, A., & Dey, P. (2023). Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism. Antioxidants, 12(4), 930. https://doi.org/10.3390/antiox12040930
Senger, D. R., Li, D., Jaminet, S.-C., & Cao, S. (2016). Activation of the Nrf2 Cell Defense Pathway by Ancient Foods: Disease Prevention by Important Molecules and Microbes Lost from the Modern Western Diet. PLOS ONE, 11(2), e0148042. https://doi.org/10.1371/journal.pone.0148042
Silva, L. A., Lopes Neto, J. H. P., & Cardarelli, H. R. (2019). Exopolysaccharides produced by Lactobacillus plantarum: Technological properties, biological activity, and potential application in the food industry. Annals of Microbiology, 69(4), 321–328. https://doi.org/10.1007/s13213-019-01456-9
Sun, C., Qiu, C., Zhang, Y., Yan, M., Tan, J., He, J., Yang, D., Wang, D., & Wu, L. (2023). Lactiplantibacillus plantarum NKK20 Alleviates High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease in Mice through Regulating Bile Acid Anabolism. Molecules, 28(10), 4042. https://doi.org/10.3390/molecules28104042
Tang, W., Xing, Z., Li, C., Wang, J., & Wang, Y. (2017). Molecular mechanisms and in vitro antioxidant effects of Lactobacillus plantarum MA2. Food Chemistry, 221, 1642–1649. https://doi.org/10.1016/j.foodchem.2016.10.124
Tian, X., Zhu, X., Wang, M., Guo, T., & Kong, J. (2023). Stimulation of Heme-Dependent Catalase Enhanced the Cytoprotective Effect of Lactobacillus plantarum against Oxidative Stress. Applied Microbiology, 3(1), 131–144. https://doi.org/10.3390/applmicrobiol3010011
Todorov, S. D., & Franco, B. D. G. D. M. (2010). Lactobacillus Plantarum: Characterization of the Species and Application in Food Production. Food Reviews International, 26(3), 205–229. https://doi.org/10.1080/87559129.2010.484113
Visic, M. (2022). Connecting puzzle pieces: Systematic literature review method in the social sciences. Sociologija, 64(4), 543–562. https://doi.org/10.2298/SOC2204543V
Wang, W., Liu, F., Xu, C., Liu, Z., Ma, J., Gu, L., Jiang, Z., & Hou, J. (2021). Lactobacillus plantarum 69-2 Combined with Galacto-Oligosaccharides Alleviates D -Galactose-Induced Aging by Regulating the AMPK/SIRT1 Signaling Pathway and Gut Microbiota in Mice. Journal of Agricultural and Food Chemistry, 69(9), 2745–2757. https://doi.org/10.1021/acs.jafc.0c06730
Wang, Z., Wu, J., Tian, Z., Si, Y., Chen, H., & Gan, J. (2022). The Mechanisms of the Potential Probiotic Lactiplantibacillus plantarum against Cardiovascular Disease and the Recent Developments in its Fermented Foods. Foods, 11(17), 2549. https://doi.org/10.3390/foods11172549
Wu, S., Chen, Y., Chen, Z., Zhou, Q., Wei, F., Li, P., & Gu, Q. (2023). Antioxidant properties and molecular mechanisms of Lactiplantibacillus plantarum ZJ316: A potential probiotic resource. LWT, 187, 115269. https://doi.org/10.1016/j.lwt.2023.115269
Xie, C., Zhang, Z., Yang, M., Cao, C., Zhou, Y., Zhu, Z., Gong, W., Xu, C., Yan, L., Hu, Z., Ai, L., & Peng, Y. (2022). Lactiplantibacillus plantarum AR113 Exhibit Accelerated Liver Regeneration by Regulating Gut Microbiota and Plasma Glycerophospholipid. Frontiers in Microbiology, 12, 800470. https://doi.org/10.3389/fmicb.2021.800470
You, T., Zhao, Y., Liu, S., & Xu, H. (2023). Lactiplantibacillus plantarum P101 Attenuated Cyclophosphamide-Induced Liver Injury in Mice by Regulating the Nrf2/ARE Signaling Pathway. International Journal of Molecular Sciences, 24(17), 13424. https://doi.org/10.3390/ijms241713424
Yu, A. O., Goldman, E. A., Brooks, J. T., Golomb, B. L., Yim, I. S., Gotcheva, V., Angelov, A., Kim, E. B., & Marco, M. L. (2021). Strain diversity of plant‐associated Lactiplantibacillus plantarum. Microbial Biotechnology, 14(5), 1990–2008. https://doi.org/10.1111/1751-7915.13871
Zare, D., Aryaee, H., Mirdamadi, S., & Shirkhan, F. (2024a). The Benefits and Applications of Lactobacillus plantarum in Food and Health: A Narrative Review. Iranian Journal of Public Health. https://doi.org/10.18502/ijph.v53i10.16698
Zare, D., Aryaee, H., Mirdamadi, S., & Shirkhan, F. (2024b). The Benefits and Applications of Lactobacillus plantarum in Food and Health: A Narrative Review. Iranian Journal of Public Health, 53(10), 2201–2213. https://doi.org/10.18502/ijph.v53i10.16698
Zhang, Z., Jin, M., Wang, K., Zhang, N., Zhang, Q., Tao, X., & Wei, H. (2021). Short-term intake of Lactiplantibacillus plantarum ZDY2013 fermented milk promotes homoeostasis of gut microbiota under enterotoxigenic Bacillus cereus challenge. Food & Function, 12(11), 5118–5129. https://doi.org/10.1039/D1FO00162K
Zhao, T., Wang, H., Liu, Z., Liu, Y., DeJi, Li, B., & Huang, X. (2023). Recent Perspective of Lactobacillus in Reducing Oxidative Stress to Prevent Disease. Antioxidants, 12(3), 769. https://doi.org/10.3390/antiox12030769
Zhao, Z., Wang, C., Zhang, L., Zhao, Y., Duan, C., Zhang, X., Gao, L., & Li, S. (2019). Lactobacillus plantarum NA136 improves the non-alcoholic fatty liver disease by modulating the AMPK/Nrf2 pathway. Applied Microbiology and Biotechnology, 103(14), 5843–5850. https://doi.org/10.1007/s00253-019-09703-4
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